Serial Dilution Definition A method used to stepwise dilute substance into solution with constant dilution factor in each step. Application Experimental sciences include biochemistry, pharmacology, and physics as well as in homeopathy. Difficulty Procedure: Medium Concept: Easy Concept Serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. Serial Dilution Besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. A tenfold dilution for each step is called a logarithmic dilution or log-dilution and a 3.16-fold (100.5-fold) dilution is called a half-logarithmic dilution or half-log dilution. Materials • Buffer used to dissolve the sample • The sample • Multiple tubes • Pipette or graduated cylinder • Stirring rod Procedure • Shake the solution by hand or use the stirring rod to swirl the solution. Make sure the solution is uniformly mixed. • Take half of the solution out to a new tube and add equal amount of buffer into it to dilute the solution concentration to half of the original solution. • Take half of the newly made solution to another new tube and add equal amount of buffer into it to dilute the solution concentration to one quarter of the original solution. • Continue the process until reaching the desired concentration of the solution. Analysis Serial dilution is a process of solution preparation. Best Answer: The variance in colonies produced. You get the colony size you need to work with. You do not want your plates to ' lawn ', do you? Serial dilution procedure only counts viable cells while other methods may count. What is the advantages and disadvantages to the serial dilution agar plate. Therefore, analysis is not necessary. References • •. Here's a paragraph about the molds that may help to infer the reason of the advantages during observation of mold colonies: 'Because the structural components of molds are very delicate, even simple handling with an inoculating loop may result in mechanical disruption of their components.The following culture technique es used to to avoid this disruption. After culturing, molds spores are deposited in the surface of the agar and incubated in a moist chamber at room temperature. Direct microscopic observation is then possible without fear of disruption or damage to the anatomical components'. It may be more beneficial in this aspect: allowing a full and healthy growth of the mold, that is, with its complete structure, then to be able to proceed to look through the microscope maybe it form, type of spore,sporangia or mycelium. In short, ensure that the mold to grow properly. Maybe it's not what you exactly were looking for but it could help you! Second Year of Microbiology bachelor's degree (University Of Puerto Rico in Arecibo) Paragraph taken of Capuccino/Sherman Microbiology. A laboratory Manual.Eight Edition. The following are some advantages of an agar plate verses a slant tube: 1. Surface area- An agar plate has a much larger surface area: a. Easier to isolate individual colonies using the streak-plate method. Evaluate the colony shape, margin and elevation. Can grow a larger number of cells. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range. Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large. The idea behind 'plate tectonics' is that the surface of our Earth is composed of large 'plates' that move on a molten base. If the plates didn't move, the pressure from trapped heat would cause violent explosions. For a small example, consider 'Old Faithful' in Yellowstone Park. It's a geyser -- a sort of natural steam valve that releases the pressure of very hot subsurface water. For a more dramatic example, consider Krakatoa. This was a huge volcanic eruption in Indonesia in 1883 that affected weather patterns all over the world. Of course, there are still problems where the plates meet. For example, the San Andreas fault, which runs under San Francisco, is actually a place where the North American and Pacific plates meet, and a little slipping along that fault line caused the great San Francisco earthquake in 1906. Because of the volcanoes formed by the subduction zones (where one tectonic plate falls and slides under an adjacent plate) gases from deep inside the earth are released. These gases probably formed our Nitrogen-Oxygen-Co2 atmosphere that is ideal for supporting life on our planet. In other areas where plates meet the plates push together and form non-volcanic mountains, providing variety in planetary ecosystems and species.
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